Total protein gradients could act across a range of cell sizes. (a) At a very high phosphatase rate (kp=100 s−1), the gradient is predicted to be appreciable in cells as small as a bacterium. (b) Animal cells and (c) oocytes/embryos can experience total protein gradients, simply by reducing the phosphatase rate constant, kp, according to the cell dimension. Diffusion coefficients DA=1 μm2s−1, DB=10 μm2s−1, kinase rate constant kk=100 μm/s, overall protein concentration 1 μM in all panels.