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. 2010 Nov 8;107(47):20251–20256. doi: 10.1073/pnas.1009021107

Fig. 4.

Fig. 4.

Pharmacokinetic profiles of pBChE and its PEGylated derivatives. (A) Unmodified pBChE was administered intravenously to mice at 2,000 units/kg (N = 10) or through a carotid catheter to GPs at 7,000 units/kg (low, N = 2) or at 14,000 units/kg (high, N = 2). (B) PEGylated BChEs were administered i.v. to mice: pBChE-5Kp (N = 10), pBChE-5Kc (N = 10), and pBChE-20K (N = 5). Plasma BChE levels were determined at the specified time points and converted to units per kilogram body weight assuming blood constitutes 7% of body weight in rodents. Control BChE levels were 100–130 units/kg in both mice and GPs. Data points represent mean ± SEM. (Insert) PEGylated pBChE was resolved by SDS-PAGE and protein bands were visualized by silver staining. Unconjugated pBChE (lane 1), or pBChE following conjugation to 5K PEG [2 h partial conjugation (lane 2), and 4 h complete conjugation (lane 3)], or 20K PEG (lane 4).