Skip to main content
. 2010 Nov 8;107(47):20376–20381. doi: 10.1073/pnas.1009176107

Fig. 2.

Fig. 2.

PGC-1α is sufficient to induce peroxisomal gene expression, protein, and number in vitro. mRNA expression of peroxisomal genes in brown fat (A), 10T1/2 (B), and FAO (C) cells infected with control or PGC-1α adenovirus for 72 h. Values were normalized using 36B4 (A) or 18S (B and C). Results are shown as the mean of three independent experiments ± SEM. (D) Western blot analysis of whole-cell lysates from 10T1/2 and FAO cells infected with control or PGC-1α adenovirus for 72 h. (E) mRNA expression of peroxisomal genes in U2OS cells infected with control or PGC-1α adenovirus for 48 h. All values were normalized using 36B4. Results are shown as the mean of three independent experiments ± SEM. (F) Differences in fluorescent peroxisomes in U2OS cells stably expressing RFP–SKL (RFP–SKL–U2OS) after 72-h infection with GFP or PGC-1α adenovirus, costained with DAPI (magnification 63×). (G) Peroxisome quantification in RFP–SKL–U2OS cells infected with GFP or PGC-1α adenovirus for 48 or 72 h. Results are shown as relative peroxisomal number per cell ± SEM. Statistical significance was determined by unpaired two-tailed Student's test. *P < 0.05, **P < 0.01, ***P < 0.001.