Fig. 3.

PGC-1α is able to induce the peroxisomal program in vivo. (A) Western blot analysis of whole-cell lysates of liver samples obtained from mice injected with GFP or PGC-1α adenovirus for 5 d. (B) Immunohistochemistry of liver cryosections of 8-wk-old male C57BL/6J mice injected with GFP or PGC-1α adenovirus for 5 d using the PMP70 and the Alexa Fluor 568 goat anti-rabbit antibodies (red) and counterstained with the nuclear dye DAPI (blue) (magnification 63×). (C) Quantification of peroxisome abundance in mouse livers injected with GFP and PGC-1α adenovirus for 5 d. Results are shown as relative number of peroxisomes per cell ± SEM. Statistical significance was determined by unpaired two-tailed Student's test. *P < 0.05.