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Phase behavior of the overtwisted 90-base-pair circular DNA chain in explicit NaCl salt. (A) Steps of the initial structure preparation are shown. (B) The degree of the superhelical twisting at various NaCl concentrations is characterized by the structural order parameter Q. See Materials and Methods for details. Our CG model predicts a pronounced phase transition at physiological conditions, c ∼ [50–200] mM.
