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. 2010 Nov 8;107(47):20405–20410. doi: 10.1073/pnas.1010386107

Fig. 2.

Fig. 2.

Meiotic DSBR is impaired and germline-specific expression of DSB repair genes is altered following BPA exposure. (A) Late pachytene nuclei from BPA-exposed germlines show elevated levels of RAD-51 (green) foci compared with control. (B) Quantitation of germ cell apoptosis. Germ cell corpses were scored in ethanol- and BPA-exposed p53/cep-1 heterozygous or homozygous worms. n = 20–23 gonad arms scored per genotype. Error bars represent SEM. *P < 0.0001. (C) Immunostaining for ATL-1 (red) and phospho CHK-1 (red) on midpachytene nuclei from dissected gonads of ethanol- and BPA-exposed worms. Between 7 and 15 gonads were analyzed for each condition. (Scale bars, 5 μm.) (D) Expression levels of a panel of genes implicated in DSB repair and DNA damage checkpoints were assayed by quantitative RT-PCR. Error bars represent SEM for three biological replicates each performed in duplicate. *P = 0.017 and **P < 0.01.