Fig. 9.

Expression of full-length DG and DG mutants in a pure DG^−/− MEpL cell line restored β-casein protein expression in response to laminin-111. Western blot of cell extracts prepared from cells infected with retroviral vector (VEC) or that encoding full-length DG (wtDG) or various β-DG cytoplasmic deletions (DEL A, B and C) and incubated with a laminin-111 overlay in the absence (–) or presence (+) of prolactin and hydrocortisone. Blots were incubated with antibodies specific for β-casein or E-cadherin (loading control), followed by HRP-conjugated secondary antibodies. Sizes of molecular mass markers are shown in kDa.