Figure 3.

NADPH oxidase inhibitor suppressed intracellular ROS production and TLR10 mRNA expression in THP-1 cells cultured in hypoxia. (A) Intracellular ROS production was examined in THP-1 cells grown in 3% O₂ for 2 days in the presence of three different ROS synthesis inhibitors; Apocynin (Apo, 100 μM), NADPH oxidase inhibitor; TTFA (100 μM), mitochondria electron transport chain complex II inhibitor; Allopurinol (Allo, 100 μM), xanthine oxidase inhibitor. After cultivation, the cells were incubated with 10 μM DCFH-DA for 15 min at 37 °C and assessed by FACS analysis; (B) A representative RT-PCR data showing TLR10 expression in THP-1 cells treated with ROS synthesis inhibitor. The cells grown in 3% O₂ for 3 days in the presence or absence of three different ROS synthesis inhibitors were harvested and examined by RT-PCR analysis. The relative expression of TLR10 was normalized by GAPDH using densitometric analysis. *P < 0.05, **P < 0.01.