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HIF-1 α mRNA expression in THP-1 cells as a function of various incubation times of ROS. A representative RT-PCR experiment showing HIF-1α expression in THP-1 cells treated with 100 μM H2O2. The cells that had been maintained at 37 °C in 20% O2 were treated with 100 μM H2O2 for 0 to 2 h as indicated below and then the cells were harvested and examined by RT-PCR.
