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. 2010 Oct 22;11(10):4114–4123. doi: 10.3390/ijms11104114

Figure 2.

Figure 2

Effect of E2 or BPA, with or without ICI-182,780 pretreatment. on cell proliferation of NS/PCs, by measuring the incorporation of the thymidine analogue BrdU. The BrdU-positive cells were immunocytochemically examined (A), and their percentage in the presence of E2 (B) or BPA (C) with or without ICI-182,780 pretreatment is shown on the ordinate. The ratios of the value for the estrogen-treated cells to that value for the control cells (ethanol-treated cells) were calculated, and are shown on the ordinate. Values are presented as the mean ± SEM (n = 4–8, different cultures). Significant differences in values between the estrogen-treated and non-treated cells (**P < 0.01; Student’s t test) and in those indicated by the brackets are shown (#P < 0.05, ###P < 0.001; Student’s t test).