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. 2010 Oct 22;11(10):4114–4123. doi: 10.3390/ijms11104114

Figure 3.

Figure 3

Effect of E2, BPA or E2-BSA, with or without ICI-182,780 pretreatment on cell proliferation of NS/PCs, by measuring the MTT assay. NS/PCs were pretreated or not with 10−6 M ICI-182,780 for 1 h, treated with E2 (A), BPA (B) or E2-BSA (C) for 24 h, and then subjected to the MTT assay. Values are presented as the mean ± SEM (n = 4–8, different cultures). Significant differences in values between the estrogen-treated and non-treated cells (***P < 0.001; Student’s t test) and in those indicated by the brackets are shown (##P < 0.01, ###P < 0.001; Student’s t test; NS, not significant).