Table 1.
Experimental design for the three inducer system and results of Set I.
| Experimental Conditions | E10a | E11 | E12 | E13 | E14 | E20 | E21 | E22 | E23 | E24 |
|---|---|---|---|---|---|---|---|---|---|---|
| Temperature (°C) | 50(0)b | 35(−) | 35(−) | 65(+) | 65(+) | 50(0) | 65(+) | 35(−) | 65(+) | 35(−) |
| Time (h) | 14(0) | 8(−) | 20(+) | 8(−) | 20(+) | 14(0) | 20(+) | 8(−) | 8(−) | 20(+) |
| Ethanol concentration (%) | 50(0) | 25(−) | 75(+) | 75(+) | 25(−) | 50(0) | 75(+) | 75(+) | 25(−) | 25(−) |
| Antibacterial activity (cycle I) (log CFU/mL) | 6.24 | 6.75 | 6.13 | 5.70 | 5.30 | 6.05 | 5.02 | 6.70 | 5.49 | 6.50 |
| Antibacterial activity (cycle II) (log CFU/mL) | 6.10 | 6.57 | 6.28 | 5.52 | 5.07 | 6.28 | 4.89 | 6.49 | 5.70 | 6.36 |
| Difference (cycle I-cycle II) (log CFU/mL) | 0.14 | 0.18 | −0.15 | 0.18 | 0.23 | 0.23 | 0.13 | 0.21 | −0.21 | 0.14 |
| Average activity (log CFU/mL) | 6.170 (a10) | 6.660 (a11) | 6.205 (a12) | 5.610 (a13) | 5.185 (a14) | 6.165 (a20) | 4.955 (a21) | 6.595 (a22) | 5.595 (a23) | 6.430 (a24) |
a
E10 to E24 = experiments;
b
Numbers in parentheses are the coded symbols of levels of the extraction conditions.