Table 5.
Experimental design for the three inducer system and results of Set III.
| Experimental Conditions | E10 | E11 | E12 | E13 | E14 | E20 | E21 | E22 | E23 | E24 |
|---|---|---|---|---|---|---|---|---|---|---|
| Temperature (°C) | 80(0) | 65(−) | 65(−) | 95(+) | 95(+) | 80(0) | 95(+) | 65(−) | 95(+) | 65(−) |
| Time (h) | 26(0) | 20(−) | 32(+) | 20(−) | 32(+) | 26(0) | 32(+) | 20(−) | 20(−) | 32(+) |
| Ethanol concentration (%) | 50(0) | 25(−) | 75(+) | 75(+) | 25(−) | 50(0) | 75(+) | 75(+) | 25(−) | 25(−) |
| Antibacterial activity (cycle I) (log CFU/mL) | 4.21 | 4.38 | 4.51 | 4.41 | 4.4. | 3.96 | 4.24 | 4.65 | 4.50 | 4.39 |
| Antibacterial activity (cycle II) (log CFU/mL) | 4.03 | 4.57 | 4.39 | 4.50 | 4.20 | 4.16 | 4.35 | 4.44 | 4.37 | 4.49 |
| Difference (cycle I-cycle II) (log CFU/mL) | 0.18 | −0.15 | 0.12 | −0.09 | 0.20 | −0.20 | −0.11 | 0.21 | 0.13 | −0.10 |
| Average activity (log CFU/mL) | 4.120 (a10) | 4.475 (a11) | 4.450 (a12) | 4.455 (a13) | 4.300 (a14) | 4.060 (a20) | 4.295 (a21) | 4.545 (a22) | 4.435 (a23) | 4.440 (a24) |
a
E10 to E24 = experiments;
b
Numbers in parentheses are the coded symbols of levels of the extraction conditions.