Table 1.
Summary of phosphorylated peptides seen specifically in 293 cells expressing FGFR1 before (F) and after induction by bFGF (FF) and those expressing ZNF198-FGFR1 (ZF). The identity of the peptides is given for each protein and the amino acid location of the modified tyrosine within that protein are shown.
| Peptides only identified in bFGF induced FGFR1 cells (FF) | ||
| Abi-1 | Y197 | NTPyKTLEPVKPPTVPNDyMTSPAR |
| BUD31 | Y97 | QGyENLCCLR |
| COPB | Y354 | DMGSCEIyPQTIQHNPNGR |
| DCBLD2 | Y715 | ATGNQPPPLVGTyNTLLSR |
| eIF5B | Y134 | PKVEMySGSDDDDDFNKLPK |
| EphA2 | Y772 | VLEDDPEATyTTSGGKIPIR |
| FGFR1 | Y605; | DLVSCAyQVAR |
| FGFR3 | Y599 | DLVSCAyQVAR |
| FLJ34658 | Y123 | LMEIFGTQCSyLLSR |
| hnRNP A1 hnRNP |
Y357 | NQGGyGGSSSSSSYGSGR |
| A2/B1 | Y336 | NMGGPYGGGNyGPGGSGGSGGYGGR |
| HSFY1 | Y175 | LKFyyNPNFK |
| HSFY1 | Y176 | LKFyyNPNFK |
| IGF2BP2 | Y40 | SGyAFVDYPDQNWAIR |
| ITGB3 | Y773 | WDTANNPLyK |
| KCTD12 | Y119 | EAEyFELPELVR |
| LDH-B | Y239 | MVVESAyEVIK |
| LOC120364 | Y300 | NQGGyGGSSSSSSYGSGR |
| LOC441495 | Y83 | AGPRDLLPSAPTPyPPGPAPSPK |
| MIA3 | Y1569 | AVSAAEEVKTyKR |
| PLXNC1 | Y1350 | VKEMyLTKLLSTKVAIHSVLEK |
| POLR1A | Y1126 | MWyELDEESRR |
| RPL38 | Y40 | VRCSRyLYTLVITDKEK |
| SDCCAG1 | Y883 | MKKMKEKyKDQDEEDRELIMK |
| SHIP-2 | Y986 | NSFNNPAyYVLEGVPHQLLPPEPPSPAR |
| SIPA1L3 | Y1141 | RPVSFPETPyTVSPAGADR |
| TLN1 | Y70 | ALDyYMLR |
| TTC2 | Y50 | DYNEAYNyYTK |
| HDLBP | Y437 | MDyVEINIDHK |
| VIM | Y61 | SLYASSPGGVyATR |
| ZFR | Y610 | RHRLQyKKKVNPDLQVEVK |
| Peptides identified in FF and ZF | ||
| CDK5 | Y15 | IGEGTyGTVFK |
| DYRK2 | Y382 | VYTyIQSR |
| DYRK4 | Y264 | VYTyIQSR |
| ENO1 | Y286 | SFIKDyPVVSIEDPFDQDDWGAWQK |
| FGFR1 | Y583 | RPPGLEyCyNPSHNPEEQLSSK |
| FGFR1 | Y654 | DIHHIDyyK |
| FGFR1 | Y653 | DIHHIDyyKK |
| PTPRA | Y798 | VVQEYIDAFSDyANFK |
| RDLP0 | Y24 | IIQLLDDyPK |
| RPS27 | Y31 | LVQSPNSyFMDVK |
| Peptides only identified in cells overexpressing FGFR1 (F) | ||
| BRF1 | Y115 | QPGGGQVNSSRyK |
| C12orf63 | Y1080 | DSyLEMALLyFHLKK |
| C12orf63 | Y1080 | DSyLEMALLyFHLKK |
| CASKIN2 | Y253 | NTyNQTALDIVNQFTTSQASR |
| Cdc2 | Y19 | IGEGTYGVVyK |
| CDK2 | Y19 | IGEGTYGVVyK |
| CDK3 | Y19 | IGEGTYGVVyK |
| CdkL5 | Y171 | NLSEGNNANYTEyVATR |
| CDV-3 | Y190 | KTPQGPPEIySDTQFPSLQSTAK |
| CENTD1 | Y473 | HSYPLSSTSGNADSSAVSSQAISPyACFYGASAK |
| DENND2C | Y195 | SLENIySEPEGQECGPSINPLPKPR |
| Dok1 | Y315 | IAPCPSQDSLySDPLDSTSAQAGEGVQR |
| Dok1 | Y449 | SHNSALySQVQK |
| GRF-1 | Y1087 | SVSSSPWLPQDGFDPSDyAEPMDAVVKPR |
| IL1F6 | Y96 | DIMDLyNQPEPVK |
| MAML2 | Y513 | IPSPSFGQQTFSPQSSPMPGVAGGSGQSKVMANyMyK |
| OCLN | Y287 | SNILWDKEHIyDEQPPNVEEWVK |
| p38-delta | Y182 | HADAEMTGyVVTR |
| Pcdhb20 | Y202 | ALDyEQEAELRLTLTAVDGGSPPKSGTTLVLIK |
| PKP4 | Y487 | NNYALNTTATYAEPYRPIQyR |
| PLCG1 | Y506 | NGILYLEDPVNHEWyPHYFVLTSSK |
| PLCG1 | Y783 | IGTAEPDYGALYEGRNPGFyVEANPMPTFK |
| RDX | Y134 | yGDYNKEIHK |
| RPL12 | Y14 | VVyLRCTGGEVGATSALAPK |
| SPTY2D1 | Y640 | yMESSWKEQQKEEAKSLR |
| SSX2IP | Y231 | DKKIAMDILNyVGRADGKR |
| Syk | Y323 | QESTVSFNPyEPELAPWAADKGPQR |
| TRAF6 | Y326 | METQSMyVSELK |
| ZO2 | Y1118 | IEIAQKHPDIyAVPIK |
| Peptides identified in F and FF | ||
| ELMO2 | Y48 | EVCDGWSLPNPEyYTLR |
| EphA7 | Y791 | VIEDDPEAVyTTTGGKIPVR |
| PSF | Y488 | FAQHGTFEyEYSQR |
| Shc1 | Y427 | ELFDDPSyVNVQNLDK |
| SUGT1 | Y317 | LFQQIySDGSDEVKR |
| TSRC1 | Y159 | SRLRDPIKPGMFGyGR |
| DLG3 | Y673 | RDNEVDGQDyHFVVSR |
| FGFR1 | Y585 | RPPGLEyCyNPSHNPEEQLSSK |
| FGFR1 | Y463 | LSSSGTPMLAGVSEyELPEDPRWELPR |
| Hrs | Y216 | VCEPCyEQLNR |
| Hrs | Y132 | VVQDTyQIMK |
| PIN4 | Y122 | FGyHIIMVEGR |
| PLCG1 | Y977 | ACyRDMSSFPETK |