Figure 2. Phasing of DMD exons and protein domains with peptides detected by LC-MS/MS.

79 exons contribute to normal, full-length dystrophin, and. phasing of protein domains generally differs from exon boundaries. Each exon or repeat is numbered according to its distance from N-terminus, and the subscript indicates the percentage of DMD patients in the cited database (Fokkema 2005) with that particular exon deletion or duplication. For LC-MS/MS results, mouse and human dystrophin sequences were aligned, and the location of the human analog of the mouse peptide shown in blue, with the indicated sequence of the detected peptide. About half of the detected tryptic peptides are identical between human and mouse and should translate to analyses of patients.