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. Author manuscript; available in PMC: 2011 Oct 8.
Published in final edited form as: Cell Stem Cell. 2010 Oct 8;7(4):508–520. doi: 10.1016/j.stem.2010.09.003

Figure 7. Comparing Rb and other cell cycle regulating proteins.

Figure 7

A) HPV16-E7-mediated increase in iPSC formation. Native IMR90 cells or IMR90 stably transduced with HPV16-E7 were transduced with OSNL and observed for iPSC formation. The error bar represents SEM (n=5). The western blot shows exogenous HA-tagged E7 expression.

B) Partial alleviation of casp8-DN mediated blockade of iPSC formation by HPV16-E7 in IMR90 cells. IMR90 cells stably transduced with casp8-DN were infected with OSNL or OSNL+E7 and observed for iPSC formation. The error bar represents SEM (n=4). The lower panel shows western blot analysis of the effects of dominant negative caspases and HPV16-E7 on Rb expression in OSNL-transduced IMR90 cells.

C) Western blot analysis of p53 and p21in OSNL-transduced IMR90 cells.

D) Effect of Rb knockdown by shRNA, HPV16 E7 transduction, and transduction of a dominant negative p53 on the frequencies of iPSC formation in OSNL-transduced IMR90 cells. Error bars represent SEM (n=3). Please see Figure S7 for additional related data.