A) HPV16-E7-mediated increase in iPSC formation. Native IMR90 cells or IMR90 stably transduced with HPV16-E7 were transduced with OSNL and observed for iPSC formation. The error bar represents SEM (n=5). The western blot shows exogenous HA-tagged E7 expression.
B) Partial alleviation of casp8-DN mediated blockade of iPSC formation by HPV16-E7 in IMR90 cells. IMR90 cells stably transduced with casp8-DN were infected with OSNL or OSNL+E7 and observed for iPSC formation. The error bar represents SEM (n=4). The lower panel shows western blot analysis of the effects of dominant negative caspases and HPV16-E7 on Rb expression in OSNL-transduced IMR90 cells.
C) Western blot analysis of p53 and p21in OSNL-transduced IMR90 cells.
D) Effect of Rb knockdown by shRNA, HPV16 E7 transduction, and transduction of a dominant negative p53 on the frequencies of iPSC formation in OSNL-transduced IMR90 cells. Error bars represent SEM (n=3). Please see Figure S7 for additional related data.