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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Genomics. 2010 Dec;96(6):362–368. doi: 10.1016/j.ygeno.2010.09.003

Figure 5. Conservation analysis of STK38L.

Figure 5

A. Pairwise percent nucleotide identities (upper right cells) and divergence (lower left cells) calculated for 5 species (D = dog, H = human, M = mouse, Z = zebrafish, C = chicken) for the STK38L complete coding sequence. The human and canine sequences are the most similar to each other, being 99.1% identical. Sequence distances were calculated using MegAlign 6.1 software (Lasergene, DNAstar, Inc.).

B. Multiple sequence alignment of the 5′ end (first 120 codons) of the canine STK38L putative protein sequence (labeled D) with that of human (H), mouse (M), zebrafish (Z) and chicken (C). The consensus sequence for the 5 species is listed above each set (1..60; 61…120) of aligned sequences. Differences among species are bold and boxed. In the consensus sequence for codons 61–120 (i.e. the lower panel), the 41 amino acids (KKL…FGE) missing from the putative protein in erd-affected dogs is boxed and shaded. Within this region, the S100B domain (KKL…GLD) is indicated in bold. The autophosphorylation residue at position 75 is labeled with an asterisk and boxed. The protein kinase domain (FES…) starts at position 90. See Supplementary Figure 2 for the corresponding analysis of the complete protein. Alignment was done using MegAlign 6.1 software (Lasergene, DNAstar, Inc.).