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. 2010 Dec;88(6):1181–1189. doi: 10.1189/jlb.0410211

Figure 6. P2X receptors contribute to enhanced IL-2 gene transcription in response to HS.

Figure 6.

(A) IL-2 mRNA expression was assessed in the presence or absence of pharmacological inhibition of P2X1 (NF023; 10 μM), P2X1 and P2X4 (TNP-ATP; 30 μM), P2X7 (A-438079; 10 μM), or the general P2 receptor inhibitor suramin (200 μM). Control cells were incubated with isotonic culture medium. (B) Jurkat cells were treated with siRNA targeting P2X1, P2X4, or P2X7 receptors or with nonsense siRNA (500 nM siRNA), and IL-2 mRNA expression was determined (#P<0.05 compared with CD3/CD28 stimulation; *P<0.05 and **P<0.01 compared with HS+CD3/CD28 stimulation; two-tailed unpaired Student's t tests; n=3).