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. 2010 Oct;24(10):3770–3781. doi: 10.1096/fj.10-160119

Figure 1.

Figure 1.

Development and initial characterization of Tie2-CYP2J2-Tr and Tie2-CYP2C8-Tr mice. A) Diagram of the Tr constructs. B, C) Expression of human CYP2J2 and CYP2C8 mRNA by quantitative RT-PCR in primary aortic and renal endothelial cells (B; n=1 endothelial cell pool/genotype group) and aorta and kidney tissue homogenates (C; n=11–12 mice/group) is significantly higher in Tr mice compared to wild-type (WT) littermates. *P < 0.001 vs. WT. D) Representative immunoblot of microsomal fractions isolated from whole-kidney homogenates from Tie2-CYP2J2-Tr (lines 0–8), Tie2-CYP2C8-Tr (lines 0–7), and WT littermates demonstrate higher CYP2J2 and CYP2C8 protein expression in Tr mice compared to WT littermates. Recombinant CYP2J2 and CYP2C8 protein are included as positive controls. E, F) Primary renal endothelial cells were isolated from Tie2-CYP2J2-Tr (lines 0–8), Tie2-CYP2C8-Tr (lines 0–7), and WT littermates (n=3 isolations/group) and stimulated with A23187. Concentrations of 11,12- and 14,15-EET and DHET (stable EET metabolite), and the regioisomer sum total, are significantly higher in Tr mice in primary endothelial cell media (E) and plasma (F) compared to WT littermates (n=5–6/group). *P < 0.05 vs. WT.