Figure 3.
MS4A2trunc localizes to the nuclear membrane rather than the cytoplasmic membrane. A) Confocal micrographs of LAD-2 cells transfected with MS4A2 (top panels) and MS4A2trunc (bottom panels) tagged with eGFP (pEGFP-N1) using nucleofection. MS4A2 localized to the cytoplasmic membrane. However, the truncated variant localized to the perinuclear region. B) Similar results were seen using lipofection of HMC-1 cells. C) A similar staining pattern was observed in HLMCs after overexpression with adenoviral transduction of untagged MS4A2 variants stained using FITC immunofluorescence. Images from Olympus FV1000 confocal laser scanning microscope analyzed using Fluoview FV10-ASW v1.6 software. View ×600 at 37°C.