Figure 4.
MS4A2trunc inhibits proliferation and initiates cell death in human mast cells. A) Transduction of MS4A2 had no effect on HLMC viability after 7 d when compared to the GFP control virus. However, transduction of MS4A2trunc leads to cell death by d 7. B) Time course study shows that HLMCs had died by 5 d after transduction of MS4A2trunc. GFP control virus had a minor effect on cell number compared to no-transduction control but did not induce HLMC death. C) In parallel, HLMC viability declined rapidly following MS4A2trunc transduction. D) In HMC-1 cells, transduction of MS4A2trunc profoundly inhibited proliferation. E) HMC-1 viability was reduced by ∼50% by d 7. F) Antiproliferative effect of MS4A2trunc in HMC-1 was also evident when measuring 3H-thymidine incorporation into DNA. Data are means ±se from 6 (A) or 4 (B–F) experiments. *P < 0.05, **P < 0.01; ANOVA with Tukey's posttest.