Figure 6.
Antiproliferative effect of MS4A2trunc in HMC-1 cells is due to G2/M phase arrest. A) Transduction of MS4A2trunc in HMC-1 cells induced an increase in the number of cells in G2/M phase when compared to control. This was accompanied by a decrease in cells in G1 phase consistent with G2 arrest. Notably, this effect was not evident with either GFP control virus or overexpression of MS4A2. Data in the table are means and se (in parentheses) of 3 separate experiments undertaken 48 h after transduction. B) No significant difference in the cell cycle was evident in HLMCs, probably attributable to very few cells progressing through to S and G2/M phases. C) No difference was found among the level of Cdk1, phosphorylated Cdk1 (Tyr15), or phosphorylated Cdk2 (Thr160) with the overexpression of either MS4A2 or MS4A2trunc in HMC-1 cells.