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Association of PKD1L3 and PKD2L1 proteins is required for their cell surface localization. HEK293T cells expressing HA-tagged PKD1L3 together with PKD2L1 or PKD2L1 deletion mutants (A) and those expressing HA-tagged C-terminal truncated PKD1L3 mutants in the presence of PKD2L1 (B) were stained with anti-HA antibodies under nonpermeabilized (top panels) or permeabilized conditions (bottom panels). Scale bar = 20 μm. Data are representative of ≥3 independent experiments.
