Figure 3.

Different E268A CLC-5 charge-displacement kinetics with permeant and impearmeant anions. A) Representative whole-cell currents recorded from both WT and E268A CLC-5 in different bath solutions as indicated. B) Charge-voltage relationships for the E268A CLC-5 Q_OFF currents recorded in bath solutions containing Cl⁻ (■), Br⁻ (●), NO₃⁻ (♦), aspartate (Asp⁻, ▴), or methanesulfonate (MeSO₃⁻, ▾). Fitted curves are Boltzmann functions. C) Gating charge z_g of the E268A Q_OFF current in each of the bath solutions, measured using the limiting logarithmic slope method. *P < 0.05 vs. Cl⁻ solution; ^#P < 0.05 vs. aspartate solution. Data are expressed as means ± se of ≥5 different cells.