Figure 2. L2a silences CD8 reporter expression on thymic, lymph node and intraepithelial (IEL) T cells.

Representative fluorescent activated cytometric analyses of T cells isolated from one (L2aWT5) of seven independent L2aWT transgenic mice and one (L2aD5) of five independent L2aD transgenic lines. Transgenic thymic (A) and lymph node (B) were stained with anti-CD4, anti-CD8α and anti-hCD2. Transgenic intestinal IELs (C) were stained with anti-CD8α, anti-CD8β and anti-hCD2. Expression of the hCD2 reporter gene on gated populations (circled in red) were analyzed and results are represented in histograms. Percentages and MFIs of hCD2 positive (>1.0%) subsets are shown.