Table 1.
Summary of L2a transgenic analyses.
| Transgene |
L2aWT |
L2aD |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Line | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 1 | 2 | 3 | 4 | 5 |
| Copy Number | 2 | >10 | 4 | >10 | >10 | >10 | >10 | >10 | 2 | 1 | >10 | >10 |
| CD8SP (%hCD2/MFI) | 80.4/69 | 1.5/47 | 0 | 5.4/42 | 2.5/49 | 1.1/91 | 0 | 85.1/46 | 23.7/26 | 0 | 25.7/89 | 74.6/63 |
| DP (%hCD2/MFI) | 80.3/58 | 0 | 0 | 0 | 0 | 0 | 0 | 79.8/93 | 66.4/29 | 0 | 25.8/45 | 41.2/33 |
| Conclusion in Thymus | Active | Sil/Var | inactive | Sil/Var | Sil/Var | Sil/Var | inactive | Active | Active | inactive | Active | Active |
| CD8+ (%hCD2/MFI) | 99.3/69 | 1.6/107 | 0 | 8.9/61 | 7.1/82 | 2.6/129 | 0.37/33 | 99.8/186 | 16.2/25.7 | 0 | 16.1/93 | 99.6/96 |
| Conclusion in Lymph Node | Active | Sil/Var | inactive | Sil/Var | Sil/Var | Sil/Var | Sil/Var | Active | Active | inactive | Active | Active |
| CD8αβ (%hCD2/MFI) | 74.2/38 | N.A. | 0 | 4.0/43 | 5.5/50 | N.A. | N.A. | 83.1/45 | 8.0/26 | 0 | N.A. | 90.3/52 |
| CD8αα (%hCD2/MFI) | 89.1/54 | N.A. | 0 | 1.5/56 | 0.46/96 | N.A. | N.A. | 95.4/112 | 14.1/25 | 0 | N.A. | 92.3/81 |
| Conclusion in IEL | Active | N.A. | inactive | Sil/Var | Sil/Var | N.A. | N.A. | Active | Active | inactive | N.A. | Active |
The copy number, percentages and MFIs of hCD2 positive (>1.0%) cells within CD8SP, DP and mature CD8 subsets are shown. For each transgenic line, at least 3 mice were tested, and no significant variation of reporter expression was observed in any transgenic line after 2–3 generations. Sil/Var: Silenced/Variegated. N.A, not analyzed.