Table 1.
Comparison of sequencing data from different cDNA libraries generated by standard TS oligo vs. iso3TS oligo
| cDNA synthesis method | Species | Avg length of read (nt) | Num. 454 reads (Mb) | Num. 2+ SMART oligos (concatamers) | % of concatamers |
|---|---|---|---|---|---|
| Standard TS oligo | Salvia divinorium | 197 | 342263 (77.6) | 70549 | 20.6 |
| Solanum lycopsericum | 207 | 336745 (70.0) | 60173 | 17.9 | |
| Solanum habrochaites | 165 | 329070 (54.4) | 38231 | 11.6 | |
| Modified TS oligo (iso3TS oligo) | Solanum habrochaites | 199 | 344190 (68.6) | 5032 | 1.5 |
| Isatis tinctoria | 345 | 970893 (335.9)* | 29734 | 3.1 | |
| Equisetum hyemale | 324 | 871579 (282.2)* | 159 | 0.02 | |
* data from 454 Titanium sequencing run