Table 2.
Quantitative PCR validation of microarray analysis
| Gene | Microarray | qPCR | qPCR |
|---|---|---|---|
| Testes Expression (Fold Change) | |||
| P relative to NP | P relative to NP | November relative to Jul | |
| AMH | -7.2 | -58.9 | -231.6 |
| ApoE | +4.8 | +2.5 | +1.2 |
| EF1α | -1.8 | -5.3 | -3.7 |
| β-Glob | -2.9 | -4.9 | +3.7 |
| Cat B | +3.2 | -2.3 | 1.0 |
| β2M | -1.7 | -3.4 | -2.1 |
| LPL | +2.7 | +3.7 | +2.9 |
| GNBP | -2.6 | -4.3 | -2.6 |
| Col 1A | -4.0 | -16.7 | +5.8 |
| Brain Expression | |||
| ApoE | -1.3 | -1.4 | |
| β-Glob | -1.5 | +1.3 | |
| IT-1 | n/a | -9.0 | |
| IT-2 | -1.5 | -9.1 | |
| MCH | +1.2 | +1.7 | |
| 02A07 | -1.3 | +1.1 | |
| 04H011 | +1.5 | +1.2 | |
| 22D02 | +1.3 | +3.0 | |
1Clone included a microsatellite