Table 4.
Primer list
| Gene | Accession | Primer species | Primer | Primer sequences |
|---|---|---|---|---|
| Anti Mullerian Hormone | AY722411 | S.salar | AMHF | ACAAGTGTTCGATCCAGACGTGAC |
| AMHR | CACTCAGTCTGCCTTGGTGTGG | |||
| Elongation factor 1 alpha | AF321836 | S.salar | EF1aF | TAAGGGCAACAGCAGTGGCAGTG |
| EF1aR | CGCATTTGTAGATCAGATGGCCG | |||
| Beta-globin | BT050121 | S.salar | BGF | CCCATGGCTGCGACAACCACTTTC |
| BGR | CAACACACTCTTCGTCGACCCTGAC | |||
| Beta-2-microglobulin | NM_001123699 | S.salar | RTB2F | GTACTTGTGCTCATTTACAGCGCGG |
| RTB2R | GCCACTCACGTGACAGATCAGGG | |||
| 1Type 1 collagen | BE518482 | S.salar | T1C1F | GAGGCAATGACCGATGGCTTC |
| T1C1R | GCGATGCTGTTCTTGCAGTGG | |||
| Cathepsin B | EG934102 | S.salar | CatBF | TGTGAGACTGGATACACACCTGGCTAC |
| CatBR | GCTCCTTCCACAGGTCCGTTCTTC | |||
| Guanine nucleotide binding protein | BT048931 | S.salar | GNBPF | GTCGCCAAAATGACCGAGCAG |
| 2GNBPR | GTCTCATCACGGGTCAACTTCCAC | |||
| Lipoprotein lipase | EG868885 | S.salar | LIPF | CGGCCCGACCTTTGAGTTTG |
| LIPR | TTGGGGTAGATGTCCACGTGGC | |||
| Apolipoprotein E | NM_131098 | D. rerio | ApoEF | TCTCTTGTGGTATTCTTTGCCCTGG |
| ApoER | GTTCAGACACATACTGCCAGAAACGG | |||
| Calponin 3 | BC053309 | D. rerio | Cal3F | GCAACTGTGATTTTACTGCAACTTTAGGAC |
| Cal3R | CAATCTTGTTTTTCACCTCCGCG | |||
| Isotocin 1 | BT049938 | S.salar | SsIT1F | AAAGCCTCAACCTCAACACATGGC |
| SsIT1R | CTGGATGGGAAAGCTAGTGCTGA | |||
| Isotocin 2 | CA063528 | S.salar | SsIT2F | TCAGTAAATGGGTGGGTGAAATAGGTG |
| SsIT2R | AGCTGCAAGTGACGCCAGGGT | |||
| 02A07 | FD425665 | S.salar | 02A07F | ACCATGTTGACAGCCAGTTTGCG |
| 02A07R | TTCCGCACTCTCAAGCTCACCAC | |||
| 04H01 | GT145175 | S.salar | 04H01F | ACTGTAGTAGTCAGCTTGTGAAGGATGC |
| 04H01R | GTAGTTCCATAGACAGAGAATGGATGCTC | |||
| 22D02 | FD425557 | S.salar | 22D02F | CAGCTGCTAATACAGGGTTATTGTTTTG |
| 22D02R | CACGTTTATGACAACTGACACGTG | |||
| 3MCH1 | M25754 | O.tshawytscha | MCH1F | AAGAGGCCGACCAGGACCTGA |
| MCH1R | ACTCAAGATGAGGCAGGACAAGATGC | |||
| MCH2 | M25755 | O.tshawytscha | MCH2F | TCCCCATCGGAAAGACGGAG |
| MCH2R | TTCAGGTTCATCCACAGGCCA | |||
| Ubiquitin | BT060103 | S.salar | UBQF | CCACAAAAAGCACCAAGCCAAC |
| UBQR | AGCTGGCCCAGAAGTACAACTGTG | |||
| Beta-actin | NM_001123525 | S.salar | ACTF | ATGGAAGATGAAATCGCCG |
| ACTR | CCCTCTTGCTCTGAGCCTCG | |||
| Succinate de-hydrogenase | NM_001141694 | S.salar | SUCDEF | GAGGGAAAGGAACAATACCATCAGACTG |
| SUCDER | GACAGCAGGTCCCAGGTACTTGTCTC | |||
1BE518482 is collagen 1A1
2GNBP: there is one mismatch here to the salmon sequence
3MCH1 and MCH2 nomenclature is based on the Chinook salmon (O.tshawytscha) MCH sequences