Table 2.
Multilocus sequence analysis
| Gene | Primer | Nucleotide sequence | Amplified fragment size (bp) | Number of alleles in L. taiwanensis strains |
|---|---|---|---|---|
| 16S rRNA | 16S-27 for | 5'-AGAGTTTGATCCTGGCTCAG | 1552 | 1 |
| 16S-1552rev | 5'-AAGGAGGTGWTCARCCGCA | |||
| recA | RecAfor | 5'-GAAAARRAYTTYGGWAARGGYKCDRTBATGCG | 740 | 0 |
| RecArev | 5'-TACATRATRTCDACTTCWSMNMSYTTRAATGG | |||
| pheS | PheSfor | 5'-KGGDCGYAAGGGTGAATTAAC | 908 | 0 |
| PheSrev | 5'-ACATCRTTWGTRTAGAARTCACGAATATC | |||
| tufF | Tuf-for | 5'-ATGGCAGAAAAAGAACATTACG | 1176 | 1 |
| Tuf-rev | 5'-AGTAACYTGACCRGCACCAAC | |||
| pyrG | PyrGfor | 5'-TTATGTTACYGAYGATGGTAC | 908 | 0 |
| PyrGrev | 5'-ACCACGWGTACCAAAACCAC |