Table 1.
Differential expression of proteins from fins by 2D-DIGE (Exp1, Exp2 and Exp3) and of their corresponding transcripts by hybridization to oligo microarrays
| Spot n° | Protein Name | Accession Number | ~MW ×10-3 | ~pI |
fold Exp1 |
fold Exp2 |
fold Exp3 |
microarray |
|---|---|---|---|---|---|---|---|---|
| SIGNALING | ||||||||
| 47 | hmgb1 | 42476233 | 16 | 7.0 | 2.4 | *3.7 | --- | 1.01 |
| 26 | gnb1 (G prot), βpolypep1 | 47087315 | 35 | 5.6 | 2.6 | 3.0 | --- | 1.03 |
| APOPTOSIS | ||||||||
| 27 | Annexin A1a | 31419751 | 40 | 6.0 | 2.3 | 9.1 | --- | 1.01 |
| 39 | Annexin 1a | 27762256 | 44 | 6.2 | 2.5 | --- | --- | N.F. |
| 0 | Annexin A5b | 41107552 | 36 | 5.2 | --- | --- | -2.0 | -1.08 |
| 0 | Annexin 5b | 160773369 | 36 | 5.3 | --- | --- | -1.9 | N.F. |
| DETOXIFICATION | ||||||||
| 34 | Glutathion S-Transferase | 18858197 | 16 | 8.2 | 11.0 | *2.7 | --- | 1.30 |
| 32 | Glutathion S-Transferase | 47086689 | 20 | 6.4 | 4.0 | *2.1 | --- | 1.30 |
| HAEMATOPOIESIS | ||||||||
| 10 | Transferrin | 51859259 | 70 | 6.9 | 16.1 | *4.6 | --- | 2.40 |
| 11 | Hemopexin | 33991748 | 65 | 6.3 | 2.5 | *3.2 | --- | 5.42 |
| 12 | Hemopexin | 33991748 | 65 | 6.5 | 3.5 | --- | --- | 5.42 |
| GLYCOLYSIS & ATP | ||||||||
| 24 | Aldolase fructose-bisphosphate | 37595414 | 44 | 8.3 | 5.7 | 8.2 | *1.4 | 1.15 |
| 45 | Triosephosphate isomerase 1b | 47271422 | 18 | 7.1 | 2.1 | *1.5 | 1.5 | 1.00 |
| 29 | GAPDH | 56718619 | 42 | 7.2 | 5.3 | 10.2 | *1.8 | 1.12 |
| 31 | GADPH | 53733367 | 40 | 8.2 | 4.2 | 8.7 | --- | 1.12 |
| 4 | GAPDH | 53733367 | 85 | 5.6 | -7.4 | --- | -*1.7 | 1.12 |
| 16 | Alpha enolase 1 | 37681795 | 57 | 6.7 | 6.0 | 17.6 | 1.8 | 1.18 |
| 28 | Ldhb Lactate dehydrogenase | 28277619 | 35 | 6.8 | 3.3 | --- | *2.2 | 1.04 |
| 33 | Carbonic anhydrase | 18858379 | 22 | 7.8 | 10.4 | --- | --- | -1.04 |
| 43 | Malate dehydrogenase | 47085883 | 40 | 7.8 | 2.1 | *1.7 | --- | 1.05 |
| 3 | ATP binding | 51571925 | 85 | 5.5 | -19.1 | --- | --- | -2.22 |
| 0 | ATP synthase | 41152334 | 24 | 7.0 | --- | --- | 1.5 | 1.05 |
| 46 | Creatine kinase | 55716037 | 15 | 6.5 | -3.4 | --- | -*1.7 | 3.16 |
| 20 | Creatine kinase | 55716037 | 48 | 6.4 | -3.9 | --- | -*2.0 | 3.16 |
| 5 | Creatine kinase | 55716037 | 90 | 5.9 | -4.0 | --- | -*2.0 | 3.16 |
| CYTOSKELETON & RELATED PROTEINS | ||||||||
| 42 | Keratin 18 | 41351240 | 57 | 5.3 | 3.2 | 11.2 | --- | 1.08 |
| 38 | Keratin type I | 50370316 | 57 | 5.6 | 2.4 | --- | --- | 1.09 |
| 13 | Type II cytokeratin | 18858425 | 59 | 5.1 | 3.2 | 1.0 | --- | 1.23 |
| 50 | Tubulin, gamma assoc protein 2 | 41056243 | 85 | 6.0 | 2.4 | --- | --- | -1.01 |
| 30 | Skeletal alpha-actin (S. aurata) | 6653228 | 38 | 7.9 | -2.7 | -2.8 | --- | -1.53 |
| 21 | Alpha-tropomyosin | 18859505 | 44 | 6.6 | --- | 3.4 | 7.2 | 1.56 |
| 0 | Alpha-tropomyosin | 55962544 | 36 | 5.0 | --- | --- | 11.7 | 1.56 |
| 15 | Transgelin | 37681953 | 49 | 5.2 | -7.4 | --- | --- | 1.49 |
| 22 | Kinesin-like protein | 50055013 | 50 | 6.7 | -2.7 | --- | --- | -2.5 |
| 41 | Myosin VIa | 10116291 | 114 | 5.9 | 2.0 | *5.1 | 7.7 | 1.05 |
| 0 | Myosin, light chain 2 | 18859049 | 22 | 5.0 | --- | --- | 7.3 | 1.30 |
| 19 | CapG | 29612467 | 48 | 5.3 | 2.8 | *7.6 | --- | -1.08 |
| 0 | Actin capping protein | 41053959 | 34 | 5.7 | --- | --- | -1.6 | 1.09 |
| 6 | Scinderin | 42542770 | 80 | 6.4 | 4.1 | --- | --- | -1.14 |
| 7 | Scinderin | 42542770 | 80 | 6.5 | 3.8 | --- | --- | -1.14 |
| 17 | Cyclase-associated protein-1 | 37725381 | 58 | 8.3 | 5.0 | --- | --- | 1.66 |
| 35 | Dynamin 1-like | 41055508 | 5 | 5.3 | 3.0 | --- | --- | 1.01 |
| 48 | Semaphorin 3Gb | 57790316 | 7 | 6.4 | 3.0 | --- | --- | 1.09 |
| 14 | CkII protein | 39645432 | 65 | 5.3 | 2.9 | --- | --- | 1.13 |
| 0 | fgf20 fin regeneration | 51571925 | 26 | 6.2 | --- | --- | 1.8 | 1.26 |
| 0 | fgf20 fin regeneration | 51571925 | 26 | 6.9 | --- | --- | 2.2 | 1.26 |
| 0 | fgf20 fin regeneration | 51571925 | 26 | 7.0 | --- | --- | 2.3 | 1.26 |
| 0 | fgf20 fin regeneration | 51571925 | 26 | 7.2 | --- | --- | 1.8 | 1.26 |
Exp1, experiment 1. Exp2, experiment 2. Exp3, experiment 3. Spot n°, number of spot selected in the 2 D gel of Exp1 (Figure 2) for mass spectrophotometry (MS) identification. 0, numbers of spots selected in 2 D gels of exp 2 or 3 (not shown). Gel spots were digested with trypsin and their peptides identified by MS. The corresponding detected proteins with a 100% of probability score of identification are shown grouped by their function. *, spots hypothetically identified by their apparent molecular weight (MW) and isoelectric point (IP) in their respective 2 D gels. ---, spots too faint to be quantified. ~MW and ~PI, apparent molecular weight (MW) and isoelectric points (IP) in the 2 D gel. Accession number of the identified protein sequences in the protein bank. Fold, calculated by the DeCyder-differential in-gel analysis software by the formula, volume of the spot in the VHSV-infected fin/volume of the spot in the non-infected fin. The spots on Figure 2 which have no corresponding description on Table 2 could not be identified by MS. Microarray differential expression data was obtained as explained in the legend of Table 3. Because most of the protein genes appeared several times in the microarray, their corresponding folds were calculated as the means for each of their data (n = 3-10). NF, not found annotated in the microarray.