Table 2.
Differential expression of transcripts from fins and organs by Q-PCR arrays.
| fold | ||||
|---|---|---|---|---|
| accession number | shortname | long name transcripts | Fins | Organs |
| complement components | ||||
| NM_001024435 | c9 | complement component 9 | 15.33 | 2.71 |
| NM_131243 | c3b | complement component c3b | 9.24 | -1.30 |
| NM_200863 | c8g | complement component 8. gamma polypeptide | 3.69 | 1.63 |
| immunoglobulin-related proteins | ||||
| NM_001034182 | sid4 | secreted immunoglobulin domain 4 | 35.99 | 1.09 |
| cluster differentiation antigens | ||||
| NM_001002363 | cd36 | CD36 antigen | 7.68 | 1.26 |
| NM_212619 | cd9 | CD9 antigen (p24) | 2.09 | 1.25 |
| interleukins | ||||
| NM_001020792 | il22 | interleukin 22 | 3.59 | -1.32 |
| NM_001020789 | il17d | interleukin 17d | 2.20 | 1.04 |
| major hystocompatibility complex | ||||
| NM_131471 | mhc1uba | major histocompatibility complex class I UBA gene | 3.01 | 1.41 |
| guanine nucleotide binding proteins | ||||
| NM_213224 | gnl2 | guanine nucleotide binding protein-like 2 (nucleolar) | 2.72 | -1.09 |
| NM_001002397 | gng7 | guanine nucleotide binding protein gamma 7 | 2.02 | 1.21 |
| Various | ||||
| NM_152980 | mst1 | macrophage stimulating 1 hepatocyte growth factor | 25.02 | -1.36 |
| NM_131607 | tradd | tnfrsf1a-associated via death domain | 2.83 | 1.07 |
| NM_001110278 | acvr2a | activin receptor IIa | 2.49 | -1.01 |
| NM_001039637 | foxp1b | forkhead box P1b | 2.37 | 1.09 |
| NM_200154 | sla/lpl | soluble liver antigen/liver pancreas antigen. like | 2.28 | 2.10 |
| NM_131000 | alcam | activated leukocyte cell adhesion molecule | 2.25 | -1.29 |
| NM_001040353 | crfb12 | cytokine receptor family member B12 | 1.68 | -3.33 |
For each experiment, 10 zebrafish were infected with 2 × 106 ffu of VHSV/ml at 14°C while other 10 zebrafish remained non-infected. RNA was extracted from the fins or organs 2-days after VHSV or mock infection, converted to cDNA and used for the AmpliTaq reaction in 384 well plates containing 2 × 186 selected immune-related zebrafish TaqMan Assays (Applied Biosystems). In each plate, 2 × 3 assays of the rplp0 gene were used for normalization. The mean and their corresponding p (t Student one tail) were then calculated from 5 experiments. The transcripts from either fins and/or organs with >2-fold and p < 0.05 changes were first tabulated and then the rest of the table was completed with their corresponding calculated fold values in organs and/or fins, respectively. Fold, expression level in VHSV-infected tissue/expression level in non-infected tissue. +, increased. -, decreased. ~, similar. Figure 3 shows the representation of means and standard errors (SE) of all the genes.