Fig. 5.

In scramble siRNA-treated sensory neurons, SEW2871 and S1P have varying capacities to augment neuronal excitability. Sensory neurons were treated with 200 nM scramble siRNA that was fluorescently labeled using our standard protocol. Recordings were obtained from a total of 16 small diameter neurons during exposure to 100 nM SEW2871 for 10 min and then to 1 μM S1P for an additional 10 min. A: results obtained from 7 of the 16 neurons wherein SEW2871 significantly increased AP firing and that S1P produced no additional effect. B: results obtained from 4 of the 16 neurons wherein SEW2871 was ineffective, but S1P did produce a significant increase in AP firing. C: 5 of the 16 neurons were not affected by either SEW2871 or S1P. The asterisks indicate a significant difference compared with the control condition with P < 0.05, ANOVA.