Table 1.

Effects of SEW2871 on excitability properties

	Untreated Neurons	S1PR1 siRNA Treated	Scramble
Resting membrane potential, mV
Control	−56.5 ± 1.7	−61.2 ± 1.3	−59.9 ± 1.4
2 min	−52.1 ± 2.2	−62.6 ± 1.8	−58.8 ± 1.5
4 min	−49.3 ± 4.2*	−63.0 ± 1.8	−56.9 ± 1.8*
6 min	−47.8 ± 4.4*	−63.2 ± 1.9	−55.1 ± 2.2*
10 min	−48.8 ± 4.5*	−62.5 ± 2.0	−51.8 ± 2.6*
Firing threshold, mV
Control	−15.7 ± 1.7	−16.5 ± 3.5	−18.6 ± 9.9
2 min	−18.2 ± 1.6	−14.8 ± 4.8	−19.3 ± 10.7
4 min	−17.1 ± 1.8	−12.8 ± 3.8	−19.7 ± 10.4
6 min	−14.3 ± 2.6	−14.1 ± 3.4	−18.4 ± 11.1
10 min	−16.1 ± 2.8	−15.1 ± 3.2	−22.7 ± 11.3
Rheobase, pA
Control	225 ± 67	493 ± 206	341 ± 166
2 min	120 ± 23	504 ± 210	280 ± 124
4 min	123 ± 27	525 ± 202	268 ± 135
6 min	111 ± 25	528 ± 198	259 ± 140
10 min	98 ± 18*	527 ± 226	196 ± 114*
Normalized rheobase
Control	1.0 ± 0	1.0 ± 0	1.0 ± 0
2 min	0.63 ± 0.11*	0.99 ± 0.04	0.76 ± 0.10
4 min	0.61 ± 0.14*	1.17 ± 0.08	0.75 ± 0.05
6 min	0.55 ± 0.10*	1.17 ± 0.05	0.72 ± 0.11*
10 min	0.53 ± 0.11*	1.12 ± 0.07	0.47 ± 0.13*
Resistance at threshold, MΩ
Control	268 ± 78	223 ± 55	296 ± 82
2 min	336 ± 71	227 ± 42	335 ± 107
4 min	354 ± 110	209 ± 43	372 ± 124
6 min	366 ± 75	206 ± 47	378 ± 105
10 min	379 ± 62	226 ± 55	406 ± 118
Normalized resistance at threshold
Control	1.0 ± 0	1.0 ± 0	1.0 ± 0
2 min	1.48 ± 0.26	1.09 ± 0.07	1.14 ± 0.09
4 min	1.45 ± 0.22	1.01 ± 0.06	1.24 ± 0.13
6 min	1.62 ± 0.26	0.96 ± 0.04	1.35 ± 0.17
10 min	1.85 ± 0.51	0.99 ± 0.04	1.65 ± 0.38

Values are means ± SE. Untreated neurons, n = 5; S1PR₁ siRNA treated, n = 11; Scramble, n = 7.

^*P < 0.05, repeated-measures ANOVA.