Figure 9. siRNA targeting of STAT3 changed the chemokine expression profile in TLR4KO MSC.

TLR4KO MSC were plated in 12-well plates at 0.5×10⁵cells·well⁻¹·ml⁻¹24 h prior to siRNA transfection. The lipofectamine-siRNA complex (100 nM) was then added on culture day 2. Medium was then changed after 2 days (day 4 in culture) of transfection. 3 days after transfection (day 5 in culture), the supernatant was collected from TLR4KO MSC either transfected with STAT3 siRNA or GAPDH siRNA and analyzed using a cytokine array assay. A: representative cytokine array blots are shown. B: Array signals from images of scanned X-ray films were analyzed and qualified as pixel densities. Data are the combination of two independent experiments. Results are mean ± SEM, n = 4/group, * p<0.05 vs. GAPDH-ablated TLR4KO group.