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. 2010 Dec 3;5(12):e14206. doi: 10.1371/journal.pone.0014206

Figure 10. siRNA targeting of STAT3 abolished cardioprotection of TLR4KO MSC.

Figure 10

One million TLR4KO MSC transfected with STAT3 siRNA or GAPDH siRNA were infused into the coronary circulation prior to global ischemia. Intracoronary infusion of TLR4KO MSC transfected with GAPDH siRNA provided greater cardioprotection compared to TLR4KO MSC transfected with STAT3 siRNA. The left ventricular function of hearts infused with GAPDH ablated TLR4KO MSC (black, n = 4) and STAT3 ablated TLR4KO MSC (hatched bars, n = 4) is presented. A: representative trace recorded from hearts infused with GAPDH or STAT3 ablated MSC. The line-graphs represent left ventricular function parameters over time including LVDP (% of equilibrium, B), + dP/dt (% of equilibrium, D), − dP/dt (% of equilibrium, F) and EDP (H). Recovery at end reperfusion is indicated in bar graph form: LVDP (C), + dP/dt (E), −dP/dt (G) and EDP (I). All results are reported as the mean ± SEM. * p<0.05 vs. GAPDH group.