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. 2010 Dec 3;5(12):e14218. doi: 10.1371/journal.pone.0014218

Figure 1. Schematic map of the T-DNA region of p35 and op-iap.

Figure 1

The p35 gene replaced the reporter gene of pBI121 through restriction with BamHI and SacI enzymes to construct an intermediate vector pBI121–p35. The op-iap gene replaced the reporter gene of pCAMBIA 1301 by restriction with BglII and BstEII termed p1301–iap. The p35 gene expression cassette obtained by Pst I and EcoR I restriction enzyme digestion was inserted into p1301-iap of the multiple cloning sites to construct the co-expression vector p1301-iap-p35.