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. 2010 Dec 3;5(12):e14225. doi: 10.1371/journal.pone.0014225

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Meek et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunohistochemical staining of targeted clone 1-B9 for Oct4 and Nanog (Magnification x100). (B) RT-PCR analysis of (1) Water blank, (2) DIA-M feeder layer, (3) rat E10.5 embryo, (4) E14Tg2a mouse ES cells, (5) RIF5.2 parental rat ES cell line, (6) 6-TG-resistant clone 1-B9, (7) 6-TG-resistant clone 2 F10, (8) 6-TG-resistant clone 3-B4 and (9) 6-TG-resistant clone 3-C10. (C) Immunostaining for Nestin and Tuj1 following 11 day monolayer differentiation protocol of 6-TG-resistant clone 1-B9 (Magnification x100). (D) RT-PCR analysis of (1) DIA-M feeder layer, (2) rat E10.5 embryo, (3) 6-TG-resistant clone 1-B9, (4) Embryoid bodies formed from clone 1-B9.