Table 3. Comparison of HPRT targeting frequency in ES cells.
Author | Species | Strain | Homology | Expt. | Cell No. | G418R | 6-TGR¶ | Frequency† |
Burdon | Rat | F344 | 4.7 kb | 1 | 2.7×106 | 58 | 1 | 0.4×10−6 |
2 | 2.7×106 | 53 | 1 | 0.4×10−6 | ||||
SD | 4.7 kb | 3 | 3.0×106 | 43 | 3 | 1.0×10−6 | ||
4* | 3.0×106 | 60 | 1 | 0.3×10−6 | ||||
Capecchi [19] | Mouse | 9.1 kb | N/A | 7.8×107 | 30000 | 32 | 0.4×10−6 | |
Smithies [18] | Mouse | 1.3 kb | N/A | 2.5×106 | 2000 | 4 | 1.6×10−6 | |
Thomson [20] | Human | 11.9 kb | N/A | 1.5×107 | 350 | 7 | 0.5×10−6 |
Selection with G418 first, followed by 6-TG selection.
Number of targeted cells per number of cells electroporated.
Electroporation with frt flanked NeoR hprt targeting vector.