Figure 2. ABC affects cell cycle progression.

(A) Cell cycle distribution of PC3 and LNCaP cells exposed to 150 µM ABC. Cells were harvested at the indicated time points, incubated with propidium iodide and DNA content was analysed by flow cytometry. The percentage of cells in each phase is reported. (ND, not done). The data are representative of five independent experiments. (B) A representative experiment of cell cycle progression in PC3 treated cells. The arrows indicate cell accumulation at G2/M phase. (C) PC3 and LNCaP cells were synchronized in early S phase by aphidicolin and cell cycle distribution was analyzed in treated (150 µM ABC) and untreated cells.