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Expression of PDE2A protein (A), NOS3 protein (B), NOS3 mRNA (C) and BNP mRNA (D). Effects on treated rats (T, ▪; n = 4) were compared with control rats (C, □; n = 4). Western blot results were normalized with GAPDH signal and expressed in percentage of untreated rat. Q-RT-PCR results were normalized with the 18S rRNA housekeeping gene and expressed as amplification folds relative to control. n.s.: not significant; *: P<0.05.
