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. 2010 Dec 3;5(12):e14229. doi: 10.1371/journal.pone.0014229

Figure 3. PPARG transcriptional repression is due to specific repressive chromatin marks and recruitment of HDAC1.

Figure 3

(A) Quantitative ChIP analysis in HCT116 cells was performed before and after the treatment with AZA and TSA alone or in combination. Native chromatin was incubated with antibodies directed against the indicated proteins. The immunoprecipitated DNA was used as template in qPCR reactions using specific primers for the PPARG promoter region *P<0.05, **P<0.01. (B) ChIP assays were carried out as described above against acetylated H3K9 and trimethylated H3K4 *P<0.05, **P<0.01 or (C) trimethylated H3K9 and H3K27 *P<0.05. The time-points for co-treatments were 72 hs for 5 µM AZA and 24 hs for 300 nM TSA, alone or in combination; CC indicates untreated control cells. Results are the mean values ± SD of three independent experiments, each performed in duplicate.