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. 2010 Dec 3;5(12):e15193. doi: 10.1371/journal.pone.0015193

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Substantia Nigra (SN) mRNA levels for Nurr1, HPRT1 and Hmbs were measured in adult Nurr1 +/+ (n = 5) and +/− mice (n = 5) by qRT-PCR. Two housekeeping genes (HPRT1 and Hmbs) did not differ between +/+ and +/− mice (panel A). For each sample, Nurr1 mRNA levels were normalized to the housekeeping gene Hmbs mRNA levels. Nurr1 mRNA levels are significantly decreased (66% of wildtype) in +/− mice compared to levels in +/+ mice (**p<0.005, Student's t-test).