Figure 2. CD94-deficient NK cells are educated and efficiently kill YAC-1 targets and MHC I-deficient splenocytes.

(A) Splenocytes from B6, CD94-deficient, CD94^Tg/–, and 129/SvJ mice were assayed for degranulation as measured by surface staining for CD107a and intracellular IFN-γ production upon stimulated with plate-bound anti-NKp46 mAb. (B) Splenocytes from CD94-deficient mice and wildtype 129/SvJ mice primed with poly I:C in vivo were assayed for NK cell-mediated cytotoxicity against the YAC-1 target cell. (C) CD94-deficient mice and B6 mice either depleted of NK cells with anti-NK1.1 mAb or treated with PBS received a mixture of CFSE-labeled wildtype and B2m^−/− B6 splenocytes. Twenty-four hours later the frequency or CFSE-labeled donor cells in the spleen that were B2m^−/−was determined by expression of H-2K^b. Data are representative of three to five experiments each.