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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Gastroenterology. 2010 Aug 27;139(6):2113–2123. doi: 10.1053/j.gastro.2010.08.040

Figure 2.

Figure 2

Notch inhibition impairs squamous differentiation of esophageal epithelia reconstituted in organotypic 3D culture

EPC2-hTERT cells were grown in 3D (A–C and E) and monolayer (D) cultures in the presence or absence of 1 μM compound E (GSI) or DNMAML1, and subjected to H&E staining in (A) and (E), IHC for Ki67 in (B) and IF for CK14 (red) and IVL (green) in (C). Ki67 labeling index in (B) was 36.7 ± 9.7 for DMSO; 32.8 ± 4.2 for GSI; 33.9 ± 5.4 for GFP and 36.8 ± 3.8 for DNMAML1 without significant difference (n=6). In (D), Western blotting determined DNMAML1 repression by doxycycline (DOX) at indicated concentrations in the Tet-Off system. DOX (−), 0 μg/ml DOX; DOX (+), 2 μg/ml DOX in (E). Scale bar, 50 μm.; Ep., epithelium; Str., stroma.