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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: J Immunol Methods. 2010 Oct 1;363(1):9–20. doi: 10.1016/j.jim.2010.09.025

Figure 3. Effect of spleen cell isolation and purification techniques on fibrocyte differentiation.

Figure 3

A and B) After removal, the spleen was treated with collagenase/DNase, and the cells were further purified through ACK lysis or density centrifugation. C and D) After removal, the spleen was passed through a 100 μm cell strainer, and the cells were further purified by ACK lysis or density centrifugation. E and F) After removal, the spleen was treated with collagenase/DNase and then passed through a 100 μm cell strainer. The cells were further purified by ACK lysis or Lympholyte-Mammal density centrifugation. For A, C, and E, the total number of cells recovered by the indicated purification technique was measured. For B, D, and F, the purified spleen cells were cultured in SFM in the presence or absence of IL-13 and M-CSF for 5 days. Cells were air dried, fixed, stained, and the number of fibrocytes was counted. Results are mean ± SEM (n=3).