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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: J Immunol Methods. 2010 Oct 1;363(1):9–20. doi: 10.1016/j.jim.2010.09.025

Figure 7. Murine and human SAP inhibit fibrocyte differentiation of cultured spleen cells.

Figure 7

Murine, ACK-treated spleen cells were cultured for 5 days at 3.5 × 105 cells per well in the (A) absence or (B) presence of IL-13 and M-CSF, and the indicated mouse and human SAP concentrations. Cells were air dried, fixed, stained, and the number of fibrocytes was counted. Results are mean ± SEM (n=3). In the absence or presence of IL-13 and M-CSF, ≥ 10 μg/ml mouse SAP or ≥ 1 μg/ml human SAP significantly inhibited fibrocyte differentiation compared to buffer control (t-test). *, p < 0.05; **, p < 0.01; ***, p < 0.001.