Skip to main content
. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Gastroenterology. 2010 Sep 15;139(6):2170–2182. doi: 10.1053/j.gastro.2010.09.002

Figure 4. Proliferation and differentiation of progenitor cells in the Pten null mice.

Figure 4

A. Immunohistochemical staining with Ki67 (brown stained nucleus) shows that mitotic activity is predominantly found in the peri-ductal region (arrows) in the liver progenitor cell niche (dotted circle) in Pm mice (middle). Arrow heads, ductal cells. Control livers contain few proliferating hepatocytes (arrow in left panel), and low mitotic activity at the portal triad (circled area, left panel). Right, quantification of ki67 positive cells. Values expressed as the mean ± SEM. * indicates values that are significantly different at p≤0.05. n=5. B. Immunohistochemical staining with keratin (brown stain) revealed an increase in ductal lineage cells associated with the expansion of hepatic progenitors. C. Identification of bi-lineage progenitor cells (arrows) coexpressing hepatocyte (HepPar-1) and cholangiocyte (keratin) markers in the Pm liver. Red, Hep-Par; Green, Keratin; Blue, DAPI.