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. Author manuscript; available in PMC: 2011 Dec 3.
Published in final edited form as: J Proteome Res. 2010 Oct 27;9(12):6242–6255. doi: 10.1021/pr1004696

Figure 6.

Figure 6

The FLAG-HA Ku86 multi-component complex is remodeled in response to DNA damage. HeLa S3 cells that expressed FLAG-HA Ku86 were subjected to gamma radiation, with complexes purified at 0, 0.5, 2, and 5 hours after exposure. (A) Aliquots of eluted proteins were resolved by SDS-PAGE and visualized by silver stain; arrows indicate molecular weight regions at which subtle changes in complex membership were observed. (B) Comparison of proteins identified in TAP-purified FLAG-HA Ku86 complexes from gamma-treated HeLa S3 cells with those found in the native complex (Figure 5, 40 frxn) revealed ≈80% reproducibility for RP-RP analysis. Analysis of iTRAQ ratios (C) for proteins identified in the context of gamma radiation indicated that protein membership in the complex decreased by ≈30% (relative to the native complex) over a time course of 6 hours after gamma irradiation.