Figure 4. Shh mRNA expression in sHip-1, omeprazole-treated, and Ctox mice.
A) RT-qPCR demonstrating Shh mRNA expression in the gastric fundus of sHip-1 mice compared to non-transgenic controls. B) Representative western blot of full-length and processed Shh protein in the gastric fundus of sHip-1 mice versus non-transgenic controls. C) Gastric acidity measured by base titration in omeprazole versus vehicle-treated mice. D) RT-qPCR of Shh, Gli-1, and Ptch-1 mRNA expression from the gastric fundus of omeprazole- versus vehicle-treated mice. E) RT-qPCR of Shh and H+/K+-ATPase-α mRNA expression from the gastric fundus of Ctox versus non-transgenic mice. Shown are the means for n = 5 mice per group +/− SEM (for Ctox mice n = 3). P-values are indicated such that * P < 0.05 and ** P < 0.01.